The 1982 publication Nucleases was the first book to survey this diverse and important group of enzymes. The new edition has been completely revised and updated. It provides a comprehensive review of all classes of nuclease, their modes of action and biological significance. The book is an invaluable source of information for investigators of DNA replication, recombination and repair and RNA processing, and for everyone interested in the rational use of nucleases as research reagents.
Table of Contents
| Preface/Front Matter |
| Stuart M. Linn, R. Stephen Lloyd, Richard J. Roberts |
| Excerpt PDF (i-viii) |
| 1 Mechanistic Principles of Enzyme-catalyzed Cleavage of Phosphodiester Bonds |
| John A. Gerlt |
| Excerpt PDF (1-34) |
| 2 Type II Restriction Endonucleases |
| Richard J. Roberts, Stephen E. Halford |
| Excerpt PDF (35-88) |
| 3 The ATP-dependent Restriction Enzymes |
| Thomas A. Bickle |
| Excerpt PDF (89-109) |
| 4 Homing Endonucleases |
| John E. Mueller, Mary Bryk, Nick Loizos, Marlene Belfort |
| Excerpt PDF (111-143) |
| 5 The Nucleases of Genetic Recombination |
| Stephen C. West |
| Excerpt PDF (145-169) |
| 6 Fungal and Mitochondrial Nucleases |
| Murray J. Fraser, Robert L. Low |
| Excerpt PDF (171-207) |
| 7 DNA Topoisomerases |
| Tao-Shih Hsieh |
| Excerpt PDF (209-234) |
| 8 Proofreading Exonucleases: Error Correction during DNA Replication |
| Myron F. Goodman, Linda B. Bloom |
| Excerpt PDF (235-262) |
| 9 Nucleases Involved in DNA Repair |
| R. Stephen Lloyd, Stuart Linn |
| Excerpt PDF (263-316) |
| 10 Artificial Nucleases |
| Dehua Pei, Peter G. Schultz |
| Excerpt PDF (317-340) |
| 11 Ribonucleases H |
| Zdenek Hostomsky, Zuzana Hostomska, David A. Matthews |
| Excerpt PDF (341-376) |
| 12 RNA Maturation Nucleases |
| Murray P. Deutscher |
| Excerpt PDF (377-406) |
| 13 Nucleases That Are RNA |
| Michael D. Been |
| Excerpt PDF (407-437) |
| APPENDIX A The Restriction Enzymes |
| Richard J. Roberts, Dana Macelis |
| Excerpt PDF (439-444) |
| APPENDIX B Some Well-characterized DNA-repair Nucleases/Glycosylases |
| R. Stephen Lloyd |
| Excerpt PDF (445-454) |
| APPENDIX C The Nucleases of Escherichia coli |
| Stuart Linn, Murray P. Deutscher |
| Excerpt PDF (455-468) |
| APPENDIX D Compilation of Commercially Available Nucleases |
| Ira Schildkraut |
| Excerpt PDF (469-483) |
| Index |
| Excerpt PDF (485-499) |