APPENDIX II: Biochemical Markers for Yeast Organelles

Abstract

The following enzymes have convenient assays that can be used to follow yeast organelles during cell fractionation.

Table
CompartmentMarker enzymeReferenceCell surfaceinvertase, acid phosphatase2, 8Plasma membranechitin synthetase, vanadate-sensitive Mg⁺⁺-ATPase4, 18Secretory vesiclesinvertase, acid phosphatase8, 14Mitochondria: outer membranekynurenine hydroxylase, porin (29-Kd protein)3, 6 intermembrane spaceL-lactate ferricyanide reductase (cytochrome b₂)1 inner membranecytochrome oxidase11 matrixfumarase15Vacuole: membraneα-mannosidase16 soluble contentsalkaline phosphatase, carboxy-peptidase Y, proteases A and B, ribonuclease17Golgimannoprotein outer chain oligosaccharide mannosyltransferase13, 5Endoplasmic reticulumNADPH cytochrome c oxidoreductase, UDP-GlcNAc-N-actylglucosaminyl dolichyl-phosphate transferase9, 10Nucleus: nucleoplasmaspartate transcarbamoylase12 nuclear envelopesame as endoplasmic reticulum (no distinctive markers yet)Cytoplasmglyceraldehyde-3-phosphate dehydrogenase7