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Structure of the Apocytochrome-b Gene and Processing of Apocytochrome-b Transcripts in Saccharomyces cerevisiae
Abstract
Cytochrome b is the only subunit of the coenzyme QH2–cytochrome c reductase complex synthesized on mitochondrial ribosomes (Weiss et al. 1973; Katan et al. 1976). Earlier studies of mit− mutants of yeast confirmed that the protein is also encoded in mtDNA (Tzagoloff et al. 1975; Slonimski et al. 1978; Haid et al. 1979). The complete sequences of the yeast gene (Nobrega and Tzagoloff 1980), bovine gene, and human gene (Anderson et al. 1981) have revealed that, although the proteins are highly homologous, the gene itself has undergone extensive evolutionary change. This is most dramatically illustrated by the presence of intervening sequences in the fungal genes and by their absence in the mammalian genes.
In this paper we describe the gene structure and some aspects of the processing of the apocytochrome-b messenger in Saccharomyces cerevisiae strain D273-10B. Of the yeast strains studied (Dhawale et al. 1981), S. cerevisiae D273-10B has the least complex gene organization, thereby making the analysis of the apocytochrome-b...
DOI: http://dx.doi.org/10.1101/0.213-223