APPENDIX I: The Nucleotide Sequence of the DNA of ϕX174 cs70 and the Amino Acid Sequence of the Proteins for Which It Codes

Abstract

A provisional sequence was determined by the plus and minus method (Sanger et al. 1977).1 It has now been completed using the termination method of Sanger et al. (1977).2 About 30 alterations have been made and therefore the numbering is somewhat different from that used in the provisional sequence.

The DNA sequence given is that of the circular viral (plus) strand. Numbering is from the unique Pst site, but the sequence is written starting at the first nucleotide after the termination of the gene-H protein. The letters in the left-hand margin indicate the proteins whose sequence is given on the corresponding line. Restriction enzyme recognition sites are indicated by underlining. The single-letter code for the restriction enzymes is as follows: A, AluI; F, HinfI; H, HhaI; M, MboII; P, PstI; Q, HphI; R, HindII; T, TaqI; Y, HapII; Z, HaeIII.

The position of the origin of viral-strand replication is based on the work of Langeveld et al. (1977).3 Other references are given in our earlier paper (Sanger et al. 1977)1 and elsewhere in this volume.

The cs70 mutation is cold sensitive and is believed to be a point mutation in the gene-F protein (C. A. Hutchison III, pers. comm.).